Synergistic Killing ofEscherichia ColiK-12 by UV (254nm) and H2O2

1 January 1988

journal article
research article
Published by Taylor & Francis in International Journal of Radiation Biology

Vol. 53 (3) , 477-488
https://doi.org/10.1080/09553008814552601

Abstract

Prior UV irradiation strongly increased the sensitivity to H₂O₂ of wild-type E. coli K-12 cells. This synergistic lethal interaction was also observed to a reduced extent in a polA mutant but was absent in uvrAuvrArecA and xthA mutants. In a recA mutant an antagonistic effect was observed. Prior H₂O₂ treatment did not sensitize the wild-type cells to UV irradiation. Alkaline and neutral sucrose gradient analysis, as well as DNA degradation studies, demonstrated that the synergism is due to the production of DNA double-strand breaks and a block of their repair. The possible mechanism of induction of such lesions is discussed.

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