The 3′-Terminal Nucleotide Sequences of Bacteriophage λ DNA
- 1 April 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (4) , 1151-1155
- https://doi.org/10.1073/pnas.70.4.1151
Abstract
Analyses of radioactive oligonucleotides in endonuclease digests of 3'-terminally labeled lambda DNA revealed the 3' terminal sequence -GTTACG for the l strand and -ACCCGCG for the r strand. These sequences, together with those previously known for the 5' cohesive ends, provide a total of 25 known base-pairs in the vicinity of the termini. When the cohesive ends are paired, the sequence between the nicks can be bisected by a 2-fold rotational axis of symmetry. Five of the first eight base-pairs, on either side of the axis, are rotationally symmetric. This symmetry may be involved in the recognition of the site by an enzyme responsible for formation of the cohesive ends.Keywords
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