Influence of Chloramine T Iodination on the Biological and Immunological Activity or the Molecular Radius of the Human Growth Hormone Molecule

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Abstract
Potential alterations of the somatotropic activity of human growth hormone (hGH) resulting from Chloramine T labelling reaction, iodination up to 2.7 atoms/molecule and indirect radiation effects, have been studied. Three 2×2 factorial assays, performed in hypophysectomized rats, failed to reveal any significant difference (P > 0.05) in true growth promoting activity between hGH and (127-I)hGH, even after storing the latter with 125-I. Similar results were obtained applying a sensitive and precise gel filtration technique for Stokes Radius determination and radioimmunoassay.