THE EFFECT OF GLUCOSONE ON THE PROLIFERATION AND ENERGY-METABOLISM OF INVITRO GROWN EHRLICH ASCITES TUMOR-CELLS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 36  (3-4) , 255-261
Abstract
Proliferation and energy metabolism of in vitro grown [mouse] Ehrlich ascites tumor (EAT) cells in the presence of glucosone, (D-arabino-3.4.5.6-tetrahydroxy-2-oxo-hexanal) a competitive inhibitor of hexokinase, were studied. Proliferation of the cells was completely inhibited by 2 mM glucosone without severely affecting viability (dye exclusion test). No phase specific arrest of cell growth was observed. Incorporation of [14C]thymidine into an acid insoluble fraction of the cells decreases to 5% of the controls within 8-10 h. Incorporation of [14C]leucine begins to slow down immediately after treatment with glucosone. The inhibitor (2 mM) reduces the lactate production of the cells by 60%, respiration by about 20%; the ATP/ADP ratio slows down from 4.75 to 3.5. The total inhibition of cell proliferation by 2 mM glucosone cannot be explained exclusively by inhibition of hexokinase activity and impairment of energy metabolism. Because of a lack of specificity, glucosone is not a suitable inhibitor for studies on the relationship between hexokinase activity and cell proliferation of tumor cells.

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