Quantitation of plastoquinone‐9 in photosystem II reaction center particles
- 17 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (2) , 203-206
- https://doi.org/10.1016/0014-5793(86)80246-0
Abstract
Quantitative analysis of PQ‐9 was performed on photosystem II reaction center particles isolated from Chlamydomonas reinhardtii. These particles show light‐driven electron transfer from secondary donor Z to primary quinone acceptor QA. If both Z and QA were PQ‐9, we would expect to find 2 PQ‐9/RC. Quantitative determinations indicate an average of 1.16 ± 0.14 PQ‐9/RC. As QA is not covalently bound to the RC (shown by QA reversible extraction and reconstitution) and as QA is known through optical spectroscopy to be a plastoquinone, we attribute the PQ‐9 to QA and conclude that Z is not a PQH2‐9.Keywords
This publication has 15 references indexed in Scilit:
- The cationic plastoquinone radical of the chloroplast water splitting complexBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Effect of inhibitors, redox state and isoprenoid chain length on the affinity of ubiquinone for the secondary acceptor binding site in the reaction centers of photosynthetic bacteriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Quinone and pheophytin in the photosynthetic reaction center II from spinach chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Optical characterization of Photosystem II electron donorsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Electron donation to Photosystem II in reaction center preparationsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- Supposition of the origin of Signal II from random and oriented chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- Isolation of Highly Active Photosystem II Particles from a Mutant of Chlamydomonas reinhardtiiEuropean Journal of Biochemistry, 1980
- Reaction kinetics for positive charge accumulation on the water side of choloplast photosystem IIFEBS Letters, 1976
- The Reduction of Plastocyanin by Plastoquinol‐1 in the Presence of ChloroplastsEuropean Journal of Biochemistry, 1976
- On the Molecular Identity of ESR Signal II Observed in Photosynthetic Systems: The Effect of Heptane Extraction and Reconstitution With Plastoquinone and Deuterated PlastoquinonePlant Physiology, 1969