A rapid purification procedure for pyruvate kinase from the hyphal fungus Aspergillus nidulans
- 1 October 1988
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 34 (10) , 1154-1158
- https://doi.org/10.1139/m88-203
Abstract
Pyruvate kinase was purified from the filamentous fungus Aspergillus nidulans with a 45–55% yield. The procedure involved dye-affinity chromatography and fast protein liquid chromatography, resulting in highly active and pure enzyme in milligram quantities within 2 days. The purified enzyme, a tetramer with a subunit molecular weight of 65 000 and an isoelectric point of 4.7, was used to determine the amino acid composition.This publication has 8 references indexed in Scilit:
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