INDUCTION OF CHROMOSOME MOTION IN THE GLYCEROL-ISOLATED MITOTIC APPARATUS: NUCLEOTIDE SPECIFICITY AND EFFECTS OF ANTIDYNEIN AND MYOSIN SERA ON THE MOTION*

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Abstract
Chromosome motion in glycerol-isolated mitotic apparatus (MA) of sea urchin [Pseudoceatrotus depressus, Hemicentrotus pulcherrimus and Anthocidaris crassispina] and starfish [Asterias forbesi] eggs was investigated with respect to nucleotide specificity and the effects of antisera against tryptic fragment (Fragment A) of flagellar dynein and starfish egg myosin. The motion was highly specific for ATP. GTP, ITP, CTP, UTP and ADP caused no displacement of the chromosomes towards the poles. The anti-Fragment A serum completely inhibited chromosome motion in the MA of the sea urchin egg, while antiserum against starfish egg myosin as well as its .gamma.-globulin fraction did not inhibit motion in isolated MA of the starfish egg, suggesting that chromosome motion depended upon dynein-microtubule but not upon myosin-actin interaction. Colchicine completely suppressed chromosome motion in vitro.