Optimized liquid-chromatographic method for fluorometric determination of urinary delta-aminolevulinic acid in workers exposed to lead.
- 1 September 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 33 (9) , 1665-1667
- https://doi.org/10.1093/clinchem/33.9.1665
Abstract
We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working linear range of urinary ALA concentration was 0.1 to 100 mg/L. In 25 lead-exposed workers, ALA values by the present method for urine correlated well with those obtained by a conventional colorimetric method (r = 0.996). The advantage of the present method for micro-determination of urinary ALA is its high sensitivity.This publication has 5 references indexed in Scilit:
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