Staining with Tetraphenylporphinesulfonate in Vivo

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Abstract
Tetraphenylporphinesulfonate (TPPS), a synthetic, nonnaturally occurring porphine derivative, was administered parenterally to tumor bearing rats and its in vivo localization was studied with fluorescence microscopy. TPPS was selectively localized in elastica and eosinophilic leukocytes, but not in other tissue sites rich in basic protein. The elastica of the aorta and medium sized arteries, as well as elastic fibers of the subendocardium, paratracheal connective tissue and bronchial walls showed the strongest red fluorescence. The intensity of fluorescence in these sites corresponded with the degree of sulfonation of the TPPS. The tumors showed moderate red fluorescence diffusely in the cytoplasm.