Low affinity of the receptor for 1α, 25‐dihydroxyvitamin D3 in the marmoset, a new world monkey
- 25 March 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 182 (2) , 385-388
- https://doi.org/10.1016/0014-5793(85)80338-0
Abstract
Circulating levels of 1,25-dihydroxyvitamin D are 10-fold higher in the marmoset, a New World monkey, than in man; to assess hormone receptors, we evaluated interactions of 1,25-dihydroxy vitamin D3 with virus-transformed lympocytes. soluble extracts of transformed lymphocytes from humans showed hormone binding with affinity and capacity similar to that of receptors for 1,25-dihydroxyvitamin D from other human tissues. However, soluble extracts of transformed lymphocytes from the marmoset showed a strikingly lower affinity for 1,25-dihydroxyvitamin D (Kd 2.2 vs 0.27 nM in marmoset vs human) and a mildly lower binding capacity (6.9 vs 16 fmolmg protein). A defective receptor for 1,25-dihydroxyvitamin D3 could account for resistance of target tissues to this hormone in the marmosetKeywords
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