Separation of Contaminating Pyrogenic Material from Commercial Bovine Serum Albumin.

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Abstract
Summary A pyrogenic material in commercial supplies of bovine serum albumin (BSA) has been identified as bacterial endo-toxin by its hyporeactivity in endotoxin-tolerant rabbits. Non-pyrogenic BSA was prepared from sterile calf serum. Using Sephadex G-100 column chromatography, the pyrogen could be separated from the protein in commercial BSA samples. Because of the widespread use of commercial BSA in immunologic and other types of biologic research, the contaminating endotoxin could modify results or lead to misinterpretation of experimental observations. The authors would like to thank Mrs. Elizabeth Desmond and Miss Dorothy Maloney for their technical work. Dr. Elisha Atkins helped with the studies of pyrogenicity and provided much helpful advice.

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