Electron Microscope Study of Length and Partial Denaturation of Rhizobium Bacteriophage DNA
Open Access
- 1 June 1973
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 11 (6) , 946-952
- https://doi.org/10.1128/jvi.11.6.946-952.1973
Abstract
By electron microscopy and melting of the DNA of some bacteriophages from the soil bacterium Rhizobium lupini , it was found that molecular weights range between 27 and 50 Mdaltons and GC contents between 53 and 62%. All DNAs studied are linear; one has exposed single-stranded terminals. Partial heat denaturation of two phage DNA permitted mapping of AT-rich sites within unique nucleotide sequences. The maps are asymmetric with respect to the midpoints of the DNA.Keywords
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