The second intron of the human galectin‐3 gene has a strong promoter activity down‐regulated by p53
- 17 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 363 (1-2) , 165-169
- https://doi.org/10.1016/0014-5793(95)00310-6
Abstract
Galectin-3 is a galactose-specific lectin which has been shown to be involved in several biological functions such as cell growth regulation, cell aggregation and cell differentiation. The partial cloning of the human genomic sequences reveals the presence of a 651 by intron, 18 by downstream of the translation initiation site. This intron contains several regulatory elements found in many eukaryotic genes. This sequence, when inserted upstream of a promoter-free luciferase gene, induces the expression of luciferase, demonstrating the promoter activity of the intron upon transfection in human or murine cells. This promoter activity is down-modulated by wild-type p53 but not by a mutated form of p53Keywords
This publication has 25 references indexed in Scilit:
- Activation of Rat Basophilic Leukemia Cells by ϵBP, an IgE-Binding Endogenous LectinCellular Immunology, 1994
- Galectins: A family of animal β-galactoside-binding lectinsPublished by Elsevier ,1994
- Genomic cloning of the gene for an IgE-binding lectin reveals unusual utilization of 5' untranslated regionsBiochemistry, 1992
- Definition of a consensus binding site for p53Nature Genetics, 1992
- Expression of carbohydrate binding protein 35 in human fibroblasts: Variations in the levels of mRNA, protein, and isoelectric species as a function of replicative competence*1Experimental Cell Research, 1991
- p53 Mutations in Human CancersScience, 1991
- Human IgE-binding protein: a soluble lectin exhibiting a highly conserved interspecies sequence and differential recognition of IgE glycoformsBiochemistry, 1990
- The Mac-2 antigen is a galactose-specific lectin that binds IgE.The Journal of Experimental Medicine, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- Identification of carbohydrate binding protein 35 in heterogeneous nuclear ribonucleoprotein complexBiochemistry, 1988