Purification of human epidermal growth factor (urogastrone) from urine
- 1 July 1987
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 166 (2) , 295-301
- https://doi.org/10.1111/j.1432-1033.1987.tb13514.x
Abstract
Human epidermal growth factor has been isolated from a concentrated chromatographic eluate of human urine. The purification method utilizes six chromatographic steps including adsorption to aminoethylcellulose (AE-11), gel filtration on Sephadex G-50, carboxymethylcellulose (CM-52) chromatography, ion-exchange HPLC and reverse-phase HPLC. The final product appears homogeneous and identical to pure .gamma.-urogastrone when analyzed by polyacrylamide gel electrophoresis and reverse-phase HPLC using two eluent systems. The yield of the method described above allowed the development of a sensitive radioimmunoassay system for this growth factor.This publication has 20 references indexed in Scilit:
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