Isozymic forms of protein kinase C in regenerating rat liver
- 24 April 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 247 (2) , 487-491
- https://doi.org/10.1016/0014-5793(89)81397-3
Abstract
The expression of multiple forms of protein kinase C (PK-C) was studied in regenerating rat liver using hydroxyapatite column chromatography. Two forms of the enzyme were found in the cytosolic as well as membrane fraction of livers from partially hepatectomized rats. The kinetic variation in the activation of these two liver isozymes by fatty acids, phosphatidylserine and diacylglycerol was similar to that reported for the PK-C subspecies from rat brain, designated types II and III. Intracellular redistribution of PK-C caused by phorbol 12-myristate 13-acetate (PMA) was concentration-dependent and was due to translocation of isozyme III, because type II was insensitive to 5 × 10−8 M PMA. The activity ratio of the two isozymes in either the particulate or cytosolic fraction was the same at 22 h as compared to 4 h after partial hepatectomy.Keywords
This publication has 33 references indexed in Scilit:
- Metabolic responsiveness to phorbol ester and activity of protein kinase C in isolated hepatocytes from partially hepatectomized ratsBiochemical and Biophysical Research Communications, 1989
- Isolation of protein kinase C subspecies from a preparation of human T lymphocytesFEBS Letters, 1988
- Synthesis of a new cell penetrating calpain inhibitor (calpeptin)Biochemical and Biophysical Research Communications, 1988
- Linoleic acid is a potent activator of protein kinase C type III- α isoform in pancreatic acinar cells; its role in amylase secretionBiochemical and Biophysical Research Communications, 1988
- Differential expression of multiple protein kinase C subspecies in rat central nervous tissueBiochemical and Biophysical Research Communications, 1987
- A single form of protein kinase C is expressed in bovine adrenocortical tissue, as compared to four chromatographically resolved isozymes in rat brainBiochemical and Biophysical Research Communications, 1987
- Three distinct forms of rat brain protein kinase C: Differential response to unsaturated fatty acidsBiochemical and Biophysical Research Communications, 1987
- Association of calpains 1 and 2 with protein kinase C activitiesFEBS Letters, 1987
- Molecular biology of liver regenerationBiochimie, 1986
- Rapid purification of protein kinase C by high performance liquid chromatographyBiochemical and Biophysical Research Communications, 1986