A Screening Method for the Detection of Erythrocyte Pyruvate Kinase Deficiency
- 1 July 1964
- journal article
- research article
- Published by S. Karger AG in Enzymologia Biologica Et Clinica
- Vol. 4 (1) , 51-57
- https://doi.org/10.1159/000458013
Abstract
A screening test is described for the evaluation of erythrocyte pyruvate kinase activity. The method is based on the use of an indicator sensitive to the variations of pH induced by the enzyme reaction. The reliability and reproducibility of the test have been compared with the spectrophotometric assay, in normal subjects, in two patients affected by hemolytic anaemia due to pyruvate kinase deficiency, and in a group of heterozygous subjects with intermediate values of pyruvate kinase activity.Keywords
This publication has 3 references indexed in Scilit:
- Hereditary Non-spherocytic Hemolytic Anemia of the Pyruvate-kinase Deficient TypeAnnals of Internal Medicine, 1963
- Pyruvate Kinase (PK) Deficiency Hereditary Nonspherocytic Hemolytic AnemiaBlood, 1962
- Autohemolysis and Other Changes Resulting from the Incubation in Vitro of Red Cells from Patients with Congenital Hemolytic AnemiaBlood, 1954