Microbial Growth Estimation in Liquid Media Exposed to Temperature Fluctuations

Abstract
Comparisons of linear, square root and Arrhenius specific growth rate‐temperature models showed no clear overall preference. At low aw and refrigeration temperature, the linear model was satisfactory for Brochothrix thermosphacta and Pseudomonas fluorescens. A linear relationship between (specific growth rate)−1 and lag phase was observed for these two psychrotrophs. The R2 for B. thermosphacta in 3% (aw= 0.968), 6% (aw= 0.950), and 9% NaCl (aw= 0.943) media was 0.865, 0.946 and 0.994, respectively. In 8% (aw= 0.973) and 20% (aw= 0.948) glycerol media, the R2 was 0.998 and 0.999, respectively. R2 for P. fluorescens growth in NaCl (3 or 4%) or glycerol (10 or 20%) media was >0.99. A cumulative growth adaptation function successfully estimated microbial growth at fluctuating temperatures.

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