A Strategy for the Development of Small-Molecule-Based Sensors That Strongly Fluoresce When Bound to a Specific RNA
- 7 October 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (43) , 14954-14955
- https://doi.org/10.1021/ja0530319
Abstract
We report a broadly applicable approach for the development of small-molecule-based RNA sensors. Our photoinduced electron transfer (PET) sensor consists of a fluorescein derivative as the fluorophore and two aniline derivatives as electron donors (quenchers). The isolation of electron-donor-binding RNA by in vitro selection (also known as SELEX) yielded an RNA aptamer that could increase the fluorescence intensity of the sensor by 13-fold. This result shows that RNA−electron-donor interactions can be used to develop modular RNA chemosensors.Keywords
This publication has 11 references indexed in Scilit:
- Effect of Binding and Conformation on Fluorescence Quenching in New 2‘,7‘-Dichlorofluorescein DerivativesOrganic Letters, 2004
- Mfold web server for nucleic acid folding and hybridization predictionNucleic Acids Research, 2003
- Improvement and Biological Applications of Fluorescent Probes for Zinc, ZnAFsJournal of the American Chemical Society, 2002
- Fluorescent Sensors for Zn2+Based on a Fluorescein Platform: Synthesis, Properties and Intracellular DistributionJournal of the American Chemical Society, 2001
- Specific Covalent Labeling of Recombinant Protein Molecules Inside Live CellsScience, 1998
- Signaling Recognition Events with Fluorescent Sensors and SwitchesChemical Reviews, 1997
- Aptamer structures from A to ζChemistry & Biology, 1996
- Chemical Communication in Water Using Fluorescent ChemosensorsAccounts of Chemical Research, 1994
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990