Preferential binding of H1e histone to GC-rich DNA
- 11 January 1994
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 33 (1) , 384-388
- https://doi.org/10.1021/bi00167a049
Abstract
We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentially and cooperatively to the GC-rich region of the DNA. A chemically synthesized peptide containing 25 residues, corresponding to a region of the carboxyl-terminal domain of H1e, shows the same sequence preference but does not exhibit cooperativity.Keywords
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