Determination of sulfite in white wine by amperometric flow injection analysis with an immobilized sulfite oxidase reactor.
Open Access
- 1 January 1989
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 53 (9) , 2347-2353
- https://doi.org/10.1271/bbb1961.53.2347
Abstract
A continuous-flow sensor, in which sulfite oxidase (SOD) was immobilized on CNBr-activated Sepharose, was developed for the determination of sulfite in white wine. Hydrogen peroxide produced by the enzyme reaction was monitored with a platinum electrode, which was covered with a dialysis membrane. The response of the sensor was linear in the range of 1 - 10 ppm sulfite, with a correlation coefficent of 0.999. The relative standard deviation for 10 injections was 2.3 % at the 5 ppm sulfite level. The gelatin coagulation procedure was introduced for the removal of interfering substances in wine such as polyphenol compounds. The method was applied to automatic sample pretreatment.Keywords
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