General Method for the Isolation of Conditional Lethal Mutants in Any Required Region of the Virus Genome: Its Application to the 'Semi-essential' Region of Phage Mu
- 1 January 1986
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 132 (1) , 79-82
- https://doi.org/10.1099/00221287-132-1-79
Abstract
A general method is presented that enables viral mutants to be isolated in any required region of their genome. An application of the method is reported relating to the isolation of conditional lethal mutants in the ''semi-essential'' region of phage Mu. Some properties are described of one mutant found in this way, Mucts62 SEEam8 Apl, which is typical of a class of mutants that were isolated.This publication has 4 references indexed in Scilit:
- Bacteriophage Mu DNA replication is stimulated by non-essential early functionsMolecular Genetics and Genomics, 1982
- Cloning of DNA fragments of the right end of phage Mu and location of the HindIII, SalI, PstI, and BamHI restriction sites on the genetic map of MuVirology, 1980
- The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistanceMolecular Genetics and Genomics, 1979
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977