Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus.
Open Access
- 1 December 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (24) , 10100-10103
- https://doi.org/10.1073/pnas.86.24.10100
Abstract
Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.This publication has 34 references indexed in Scilit:
- Cellular receptor for poliovirus: Molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamilyCell, 1989
- Identification and sequence of the gene encoding gpIII, a major glycoprotein of varicella-zoster virusVirology, 1987
- beta2 Microglobulin Enhances the Infectivity of Cytomegalovirus and When Bound to the Virus Enables Class I HLA Molecules To Be Used as a Virus ReceptorJournal of General Virology, 1987
- Cytomegalovirus Strain AD169 Binds beta2 Microglobulin in Vitro after Release from CellsJournal of General Virology, 1987
- The properties and sequence of glycoprotein H of herpes simplex virus type 1Virology, 1986
- Idiotypic Mimicry of Biological ReceptorsAnnual Review of Immunology, 1986
- Isolation and biochemical characterization of the mammalian reovirus type 3 cell-surface receptor.Proceedings of the National Academy of Sciences, 1985
- Murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus.Proceedings of the National Academy of Sciences, 1984
- Virus-Specific IgG and IgM Antibodies in Normal and Immunocompromised Subjects Infected with CytomegalovirusThe Journal of Infectious Diseases, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970