The Isoelectric Point of the p30 Polypeptide as a Marker of Mouse Endogenous Viruses

Abstract
The isoelectric point (pI) of the p30 polypeptide of members of the 3 known classes of mouse C-type endogenous viruses was determined by column and by thin-layer gel isoelectric focusing. Each class was characterized by a particular variant of p30 (isop30), with pI values of 6.1 for class I (ecotropic), 5.7 for class II (xenotropic) and 5.5 for class III (NZB, NIH, ATS124, also xenotropic). The 6.1-isop30 was found as a minor component of rat-grown NZB virus and of a number of laboratory strains of mouse C-type viruses.

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