ANTI-CARBOHYDRATE PRECIPITINS AND HEMAGGLUTININS IN HEMOLYMPH FROM TRIDACNA MAXIMA (RODING)

  • 1 January 1975
    • journal article
    • research article
    • Vol. 150  (4) , 354-363
Abstract
Hemolymph from the elongate clam, T. maxima readily precipitates with H-blood group substances, pneumococcus type XIV polysaccharide, human milk and salivas, and with a number of polysaccharides which contain the O-.beta.-D-galactopyranosyl-(1-6)-D-galactose structure. Precipitation was demonstrated using gel diffusion and quantitative precipitin methods. T. maxima hemolymph strongly agglutinates human erythrocytes, and hemagglutination can be inhibited by the same preparations which precipitate with the clam extract. Precipitins and hemagglutinins are inhibited by N-acetyl-D-galactosamine and by D-galactose residues preferably in .beta.-linkage. After agar gel immunoelectrophoresis at pH 8.6, T. maxima precipitin arcs are found in the .alpha.-region. Precipitation and inhibition results suggest that T. maxima extract and purified precipitin, may find widespread application in the study many biologically important carbohydrates and glycoproteins.

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