Temperature-inducible amber suppressor: construction of plasmids containing the Escherichia coli serU- (supD-) gene under control of the bacteriophage lambda pL promoter

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Abstract
An E. coli DNA fragment containing the structural gene serU132 for the nonsense suppressor .**GRAPHIC**. was identified and purified by being cloned into a plasmid vector. Information obtained from DNA sequence analysis was used to select a serU132 fragment for insertion downstream from the bacteriophage .lambda. pL promoter in 2 pBR322-.lambda. derivatives. In nonsense mutant strains bearing the resulting ser U132 hybrid plasmids, the presence of the .lambda. cI857 repressor gene carried on the same plasmid or in a prophage genome permits thermal regulation of suppressor synthesis.