ANTIBODIES IN THE PIG AGAINST PIG INSULIN
- 1 November 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 47 (3) , 367-370
- https://doi.org/10.1530/acta.0.0470367
Abstract
Sera from three pigs injected subcutaneously with respectively pig, ox, and horse insulin for 87 days were incubated with 131I-pig insulin and investigated for the presence of antibodies by means of immunoelectrophoresis and autoradiography. A binding of 131I-pig insulin to the gamma globulin fraction could be demonstrated in all animals after the commencement of the injections. Thus a cross reactivity between pig and ox insulin and pig and horse insulin occurred, however, apparently to a lesser degree in the latter case. The presence of a 131I-pig insulin binding gamma fraction in the serum from the animal injected with pig insulin, indicates a formation of antibodies against pig insulin in the pig.Keywords
This publication has 3 references indexed in Scilit:
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJournal of Clinical Investigation, 1960
- Heterogeneity of Insulin-Antibody Complexes in Rabbits and Guinea PigsExperimental Biology and Medicine, 1960
- Species differences in insulinArchives of Biochemistry and Biophysics, 1956