A Referee Blood Experiment Involving the Use of Microchemical Methods

Abstract
As a part of the Cooperative Nutritional Status Studies in the Northeast Region (Project NE4, '47–'52) a referee study was conducted to measure the variation in results of microchemical analyses of blood serum for ascorbic acid, vitamin A and carotene. Variations in replicate determinations within a station were found to be small compared with station-to-station variation. For each nutrient there was a tendency for the stations to follow a pattern, in which those reporting high values for one blood tended to report high values on the others and vice versa. Evidence is given that the station differences were similar in magnitude to the day-to-day fluctuations in blood levels of ascorbic acid and carotene, as estimated from subsequently obtained data. Probable sources of station-to-station variation are discussed and suggestions given for conducting future referee studies of this type.

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