Cytokine gene expression in epidermis with biological effects following injection of naked DNA
- 1 June 1995
- journal article
- research article
- Published by Springer Nature in Nature Genetics
- Vol. 10 (2) , 161-166
- https://doi.org/10.1038/ng0695-161
Abstract
The epidermis is readily accessible for genetic manipulation and is easily monitored. Using pig skin because it is very similar to human skin morphologically, we have developed a method to transiently express biologically active factors in epidermis. Following direct injection of naked plasmid DNA into skin, DNA is taken up and transiently expressed at high levels by epidermal keratinocytes. Injection of interleukin–8 plasmid DNA into skin results in the appropriate biological response of neutrophil recruitment, demonstrating functional utility. In addition to this model's therapeutic uses, the biological effects of structural gene products on the epidermis could also be studied in vivo.Keywords
This publication has 34 references indexed in Scilit:
- DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations.Proceedings of the National Academy of Sciences, 1993
- Keratinocyte Gene TherapyArchives of Dermatology, 1993
- Cancer vaccinesImmunology Today, 1993
- Towards gene therapy for haemophilia B using primary human keratinocytesNature Genetics, 1993
- Molecular genetics of the cutaneous basement membrane zone. Perspectives on epidermolysis bullosa and other blistering skin diseases.Journal of Clinical Investigation, 1992
- Genetic immunization is a simple method for eliciting an immune responseNature, 1992
- The InterferonsJAMA, 1991
- Long-term transplantation of canine keratinocytes made resistant to G418 through retrovirus-mediated gene transfer.Proceedings of the National Academy of Sciences, 1990
- Systemic distribution of apolipoprotein E secreted by grafts of epidermal keratinocytes: implications for epidermal function and gene therapy.Proceedings of the National Academy of Sciences, 1989
- Expression of an Exogenous Growth Hormone Gene by Transplantable Human Epidermal CellsScience, 1987