Fatty Acid Synthesizing Systems in Chick Liver: Influences of Biotin Deficiency and Dietary Fat

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Abstract
Subcellular fractionations were carried out with liver homogenates obtained from chicks fed a control or biotin-deficient diet or the same diets with 10% added fat for 3 weeks posthatching. Through the use of marker enzymes, it was found that the activities of the lipogenic enzymes, acetyl CoA carboxylase, fatty acid synthetase and the mitochondrial and microsomal systems of fatty acid elongation, could be measured independently in the homogenates and in the subcellular fractions isolated from the homogenates. The influence of diet on body weight, liver weight and the specific activities of the lipogenic enzymes was measured also. Biotin deficiency depressed 3-week body and liver weights, but dietary fat had no effect. Biotin deficiency reduced acetyl CoA carboxylase activity, but did not affect the activities of fatty acid synthetase, the microsomal system and the mitochondrial system. Dietary fat depressed all lipogenic enzymes except the mitochondrial system. These data are advanced to support the hypothesis that dietary fat can mask the effects of biotin deficiency on in vivo synthesis of fatty acids.

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