Feline Leukemia Virus: Purification From Tissue Culture Fluids2

1 September 1970

journal article
research article
Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute

Vol. 45 (3) , 499-503
https://doi.org/10.1093/jnci/45.3.499

Abstract

Purified infectious virus from supernatants of feline leukemia tissue culture was prepared by continuous-flow centrifugation followed by isopycnic banding in sucrose. Thin sections showed a particle having a diameter of 110 mμ. A minimum of 100 μg nucleic acid per 1.5 liters of supernatants was recovered from the preparations.

Keywords

LEUKEMIA VIRUS
FELINE
TISSUE CULTURE
BODY FLUID
CENTRIFUGATION
VIRUSES
DIAMETER
FLUID FLOW
LEUKEMIA
NUCLEIC ACIDS
SUCROSE