Marking Horn Flies with Fluorescent Dyes and Other Materials12

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Abstract
Of several techniques tested for marking large numbers (5000–12,500) of Haematobia irritans (L,), the use of 1% solutions of fluorescent dyes sprayed uniformly and rapidly onto chilled flies by a mechanized technique was most satisfactory. In both laboratory and large-screen cage tests, these dyes were retained for more than 2 weeks by nearly all the flies. The most satisfactory colors were Fire Orange® and Are Yellow® followed by Saturn Yellow® and Horizon Blue® because these 4 colors could all be distinguished on the flies. The dyes and technique were used successfully to mark the millions of flies in a horn fly suppression program in Hawaii. Mortality of marked flies did not differ significantly from that of unmarked flies. Fluorescent dusts or pH indicators applied as sprays were not as well retained as the sprayed fluorescent dyes.

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