Prenatal Determination of Fetal RhD Type
- 17 March 1994
- journal article
- letter
- Published by Massachusetts Medical Society in New England Journal of Medicine
- Vol. 330 (11) , 795-796
- https://doi.org/10.1056/nejm199403173301122
Abstract
In the August 26 issue of the Journal, Bennett and colleagues describe the application of the polymerase chain reaction to determine the RhD genotype of fetuses by using DNA derived from chorionic villi and amniocytes1. The first set of oligonucleotide primers used (A1 and A2) yields a 136-base pair (bp) product common to the RhCcEe and RhD genes, whereas the second set (A3, a consensus primer, and A4, a primer specific for the 3' noncoding sequence of the RhD gene) yields a 186-bp product only in RhD-positive subjects.Keywords
This publication has 4 references indexed in Scilit:
- Prenatal Determination of Fetal RhD Type by DNA AmplificationNew England Journal of Medicine, 1993
- Molecular cloning of RhD cDNA derived from a gene present in RhD- positive, but not RhD-negative individualsBlood, 1993
- Molecular cloning and primary structure of the human blood group RhD polypeptide.Proceedings of the National Academy of Sciences, 1992
- cDNA cloning of a 30 kDa erythrocyte membrane protein associated with Rh (Rhesus)-blood-group-antigen expressionBiochemical Journal, 1990