A Spectrophotometry Analysis of Tissue Staining

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Abstract
By comparing spectral absorption curves of representative staining solns. and of substances stained with these solns. it is shown that information may be obtained regarding chemical changes associated with the staining process. The stains used in these detns. were acid fuchsin, anilin blue, azocarmine G, basic fuchsin, eosin Y, orange G, picric acid and Sudan IV. The substrates stained were gelatin, tendon, blood plasma, thymus gland and fat. Aqueous basic fuchsin and fuchsin-sulfurous reagent to which formalin was added (Schiff reaction) are different stains. The spectral absorption curves for staining solns. and substances stained with the solns. were comparable. Within the limitations of the spectrophotometric methods and stains employed, there was no evidence of significant chem. alteration in the chromophore radicals of the stains associated with the process of tissue staining.

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