Up-regulation of MDC15 (metargidin) messenger RNA in human osteoarthritic cartilage
Open Access
- 1 September 1999
- journal article
- basic science
- Published by Wiley in Arthritis & Rheumatism
- Vol. 42 (9) , 1946-1950
- https://doi.org/10.1002/1529-0131(199909)42:9<1946::aid-anr21>3.0.co;2-e
Abstract
Objective The aim of the study was to investigate the messenger RNA (mRNA) expression of the disintegrin metalloproteinase MDC15 (metargidin, or ADAM-15) in normal and osteoarthritic (OA) articular cartilage. Methods In situ hybridization experiments and reverse transcription–polymerase chain reaction (RT-PCR) were performed on tissue samples of adult normal and OA articular cartilage. Results MDC15 mRNA could be detected in normal articular cartilage by RT-PCR using tissue-extracted total RNA as a template. However, the mRNA level remained below the sensitivity of in situ hybridization. In contrast, in situ hybridizations of OA cartilage revealed an intense staining with the MDC15-specific riboprobes. The extension of the analysis to chondrosarcomas showed a strong up-regulation of MDC15 mRNA in these malignant transformed cells. Conclusion Our results demonstrate a markedly strong up-regulation of MDC15 in adult OA and neoplastic cartilage compared with adult normal articular cartilage, indicating a potential role of the disintegrin metalloproteinase in cartilage remodeling.Keywords
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