The ATP Synthetase of Escherichia coli K12: Purification of the Enzyme and Reconstitution of Energy-Transducing Activities
- 1 October 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 100 (1) , 175-180
- https://doi.org/10.1111/j.1432-1033.1979.tb02046.x
Abstract
The ATP synthetase of E. coli K12 was purified by a simple procedure. The dicyclohexylcarbodiimide-sensitive ATPase activity was enriched 21-fold. The ATP synthetase preparation contained the 8 polypeptides (.alpha., .beta., .gamma., a, .delta., b, .epsilon., c) of the enzyme and a residual contamination (4% of the total protein) as shown by dodecylsulfate/polyacrylamide electrophoresis. The polypeptide c was specifically labeled with [14C]dicyclohexylcarbodiimide. Energy-transducing activities were reconstituted from soybean phospholipids and the purified enzyme. The proteoliposomes exhibited a significantly higher ATP-32Pi exchange activity and a higher proton-translocating activity as compared to the untreated membranes.This publication has 34 references indexed in Scilit:
- Reconstitution of oxidative phosphorylation by chemically modified coupling factor F1: differential inhibition of reactions catalyzed by F1 labeled with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole or 2,3-butanedioneBiochemistry, 1979
- Reconstitution of the energy transformer, gate and channel subunit reassembly, crystalline ATPase and ATP synthesisBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1978
- Identfication of the altered subunit in the inactive F1ATPase of an Escherichia coli uncA mutantBiochemical and Biophysical Research Communications, 1978
- Biogenesis of mitochondrial ATPaseBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1977
- ATP-dependent proton translocation and quenching of 9-aminoacridine fluorescence in inside-out membrane vesicles of a cytochrome-deficient mutant of Escherichia coliBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- The Use of Several Energy‐Coupling Reactions in Characterizing Mutants of Escherichia coli K12 Defective in Oxidative PhosphorylationEuropean Journal of Biochemistry, 1976
- ATPase of Escherichia coli: purification, dissociation, and reconstitution of the active complex from the isolated subunitsBiochemistry, 1976
- Purification and characterization of a dicyclohexylcarbodiimide-sensitive adenosine triphosphatase complex from membranes of Escherichia coliBiochemical and Biophysical Research Communications, 1975
- Purification of a factor for both aerobic‐driven and ATP‐driven energy‐dependent transhydrogenases of Escherichia coliFEBS Letters, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970