Synthesis and Properties of DNA Dumbbells Containing Chemically Active Substituted Pyrophosphate Internucleotide Groups

1 June 1996

journal article
research article
Published by Taylor & Francis in Nucleosides and Nucleotides

Vol. 15 (6) , 1237-1251
https://doi.org/10.1080/07328319608007390

Abstract

DNA dumbbells with substituted pyrophosphate groups at a definite position of the sugar-phosphate backbone were synthesized by condensation of terminal 5′-phosphomonester- and 3′-methylphosphodiester groups in nicked dumbbells. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide was used as a condensing agent. An efficient method for producing extended oligonucleotides carrying an O-methyl-substitued 3′-phosphate group was developed. Properties of the modified DNA-dumbbells were investigated. The substituted pyrophosphate group in the DNA dumbbells was efficiently cleaved under the action of N-methylimidazole or ethylendiamine aqueous solutions at pH 8.0.

Keywords

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