DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.
- 1 June 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (6) , 1887-1891
- https://doi.org/10.1073/pnas.73.6.1887
Abstract
Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phiX RFII. The reaction was inhibited by nalidixic acid and novobiocin but not by rifampicin. DNA synthesis required the phiX174 gene A product and E. coli gene products dnaB, dnaC(D), dnaG, and rep.This publication has 22 references indexed in Scilit:
- Replication of ϕA and ϕX174 in Escherichia coli mutants thermosensitive in DNA synthesisMolecular Genetics and Genomics, 1975
- Novobiocin—A specific inhibitor of semiconservative DNA replication in permeabilized Escherichia coli cellsJournal of Molecular Biology, 1975
- Multienzyme Systems of DNA ReplicationScience, 1974
- Growth of phages λ, ØX174, and M13 requires the dnaZ (previously dnaH) gene product of Escherichia coliBiochemical and Biophysical Research Communications, 1974
- Conversion of ϕX174 and fd Single-Stranded DNA to Replicative Forms in Extracts of Escherichia coliProceedings of the National Academy of Sciences, 1972
- Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coliJournal of Molecular Biology, 1971
- Fate of parental ∅X174-DNA upon infection of starved thymine-requiring host cellsVirology, 1971
- Preparation and purification of ФX-RF component IBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- The process of infection with bacteriophage φX174Journal of Molecular Biology, 1967
- THE ABORTIVE REPLICATION OF ΦX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coliProceedings of the National Academy of Sciences, 1967