A Rapid Stability-Indicating HPLC Assay for the Arabinosylcytosine Prodrug, Cyclocytidine

Publisher Website
Google Scholar
Abstract
Hydrolysis of cyclocytidine in aqueous solutions produced arabinosylcytosine which, in some cases, further reacted to form arabinosyluracil. No other degradation products were detected. A rapid isocratic reverse-phase HPLC assay for all three components in mixtures arising from cyclocytidine hydrolysis was developed. The analysis employs a 4.6 cm column together with a low methanol mobile phase containing 1-heptane sulfonic acid at pH 2.9. The ion-paring of cyclo-C, a cation, was independent of pH. However, ion-paring of arabinosylcytosine was controlled by adjusting the pH to 2.9 which is below its pKa of 4.2. The retention time of neutral arabinosyluracil (pKa = 9.2) was not affected by either the pH or the ion-pairing agent. Its separation was achieved by using a primarily aqueous mobile phase with the minimum methanol required for the other components. The time courses for cyclocytidine and its hydrolysis products were successfully defined under a variety of aqueous conditions.

This publication has 4 references indexed in Scilit: