A modified aluminum-formaldehyde (ALFA) histofluorescence method for enhanced visualization of central techolamine.

Abstract
We designed a modification of the aluminum-formaldehyde (ALFA) histofluorescence method based on preperfusion with buffer containing formaldehyde (FA). This modified ALFA technique enables visualization of all catecholamine (CA) neuron systems with a sensitivity comparable to that of the previously published glyoxlic acid (GA)-Vibratome method, providing better reproducibility and facility of the procedure than the ALFA method.