Active oxygen acts as a promoter of transformation in mouse embryo C3H/10T1/2/C18 fibroblasts.
- 1 April 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (7) , 2085-2087
- https://doi.org/10.1073/pnas.81.7.2085
Abstract
There is much evidence from in vivo and in vitro carcinogenesis studies that active oxygen species play a role in tumor promotion. Whether superoxide produced extracellularly by xanthine-xanthine oxidase (X-XO) has the capacity to promote initiated mouse embryo C3H/10T1/2 fibroblasts was tested. Cell cultures initiated with either 137Cs .gamma.-rays or benzo[a]pyrene diol epoxide I were found to transform 3-30 times more effectively when subsequently treated daily for 3 wk with nontoxic doses of X-XO. Scavengers of active oxygen radicals such as superoxide dismutase or superoxide dismutase in combination with catalase reduced the frequency of appearance of transformed foci by 3-25 times when compared to cultures receiving X-XO alone. Active oxygen species such as superoxide and H2O2 can act in a promotional manner that mimics the effects of the mouse skin promoter phorbol 12-myristate 13-acetate in this system. X-XO also acted as a weak complete carcinogen.This publication has 33 references indexed in Scilit:
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