The Behaviour of Acid-fast Bacteria in Oil and Water Systems

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Abstract
The work of Mudd on the behavior of acid-fast bacteria in oil and water systems was confirmed, using a macroscopic, in place of his microscopic, method. On shaking watery suspensions of acid-fast bacteria with oil they readily pass into the oil, while non-acid-fast organisms remain in the water. The same reaction occurs with a variety of fat solvents. The partition is complete for tubercle bacilli and 70-90% for 6 other acid-fast spp. Homologous immune serum inhibits the partition in proportion to its concentration. Emulsifying agents have a similar effect.

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