Constituents of the Cotton Bud. III. Factors that Stimulate Feeding by the Boll Weevil12

Abstract
Components of the cotton bud that stimulate feeding by the boll weevil Anthonomus grandis Boheman, were fractionated. Studies with different solvents and column chromatography provided evidence that 3 cotton-bud components having diverse chemical characteristics were present and elicited response from the weevil. Response of the insect was modified by factors such as pH, light, the diet fed, both in pre-emergence and adult stages, along with age and sex of insect. Secondary responses to a gossypolglucose incubate in the presence of emulsin, pH 8.0 phosphate buffer, or ATP, as well as sugars, salts, and ascorbic acid, were demonstrated. The insect-feeding threshold, determined from the straight-line plot of feeding activity vs. the log of extract concentration, was 0.015 mg.

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