The role of recombinant human bone morphogenetic protein-2 in PLGA capsules at an extraskeletal site of the rat
- 3 February 1999
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 45 (1) , 36-41
- https://doi.org/10.1002/(sici)1097-4636(199904)45:1<36::aid-jbm5>3.0.co;2-i
Abstract
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-β (TGF-β) superfamily and has strong bone-inductive activity in vivo. To examine the role of BMP-2 in an extraskeletal site of rat using a controlled release system of peptides, we encapsulated the recombinant human BMP-2 (rhBMP-2) with poly(DL-lactide-co-glycolide) (PLGA) and implanted the rhBMP-2/PLGA capsules in the subcutaneous area of rats. Upon histochemical examination, it was found that bone-inducing cells having alkaline phosphatase (ALP) activity appeared around the capsules by the suitably released rhBMP-2. In addition, the temporal histological examination showed that direct bone formation without cartilage occurred in the process of this ectopic bone induction. These data indicate that the role of rhBMP-2 in the extraskeletal site of rats is to induce the differentiation of mesenchymal cells into the osteoblasts.© 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 45, 36–41, 1999.Keywords
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