TheTRP4gene ofSaccharomyces cerevisiae: isolation and structural analysis
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (16) , 6357-6373
- https://doi.org/10.1093/nar/14.16.6357
Abstract
The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing information for a polypeptide of amino acids and the 5′;- and 3′-flanking regions was sequenced. The TRP4 transcript was identified and mapped with S1 nuclease. Homologies to two prokaryotic genes encoding the same function, and sequences potentially involved transcription start and termination and in regulation of TRP4 gene expression are discussed.Keywords
This publication has 30 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Nucleotide sequence of the Bacillus subtilis trpE and trpD genesGene, 1984
- THE PATHWAY OF EUKARYOTIC mRNA FORMATIONAnnual Review of Biochemistry, 1983
- A short nucleotide sequence required for regulation of HIS4 by the general control system of yeastCell, 1983
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- Transcription of the his3 gene region in Saccharomyces cerevisiaeJournal of Molecular Biology, 1981
- Attenuation in the control of expression of bacterial operonsNature, 1981
- Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experimentsGene, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Anthranilate SynthetasePublished by Wiley ,1973