CHROMATOGRAPHIC PROPERTIES OF GAMMA GLOBULIN: BEHAVIOR OF SERUM GAMMA MACROGLOBULINS*

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Abstract
Gamma globulin has been separated on DEAE cellulose into components which differ in molecular size. When sera from patients with liver disease, lymphosarcoma, primary amyloidosis, multiple myeloma, and macroglobulinemia were studied, the appearance of the chromatographic pattern of the serum on DEAE cellulose in conjunction with paper electrophoresis made it possible to detect the presence of increased amounts of macroglobulins. It has been possible in this way to differentiate between the chromatographic patterns of diseases with ordinary hypergammaglobulinemia (including multiple myeloma) and macroglobulinemia. The chromatographic method employed offered a means of preparing 7 S gamma globulin free of heavier molecular species. In the case of the rheumatoid factor, the 19 S gamma globulin has been prepared free of the 7 S variety.