In Situ Hydration of Enzymes in Non-Polar Organic Media Can Increase the Catalytic Rate
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 10 (1-4) , 269-277
- https://doi.org/10.3109/10242429409065236
Abstract
A comparison of the initial catalytic rate was made between enzyme equilibrated with water through the vapour phase and enzyme equilibrated whilst suspended in n-hexane at fixed water activities, aw. The rate of enzymic catalysis below aw = 0.58 is essentially the same for the two equilibrated systems. However at water activities greater than 0.58 it was found that enzyme hydrated in the presence of solvent showed a significant and reproducible rate enhancement. We have shown that this enhancement is not because of differences in the number of adsorbed water molecules. The possibility that it arises from either variations in the distribution of active conformations or microscopic variations in the protein powder is discussed.Keywords
This publication has 14 references indexed in Scilit:
- Activation of enzymes in organic media at low water activity by polyols and saccharidesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
- Organic solvents strip water off enzymesBiotechnology & Bioengineering, 1992
- Enzymatic catalysis and dynamics in low-water environments.Proceedings of the National Academy of Sciences, 1992
- Selective acylation of peptides catalyzed by lipases in organic solventsJournal of the American Chemical Society, 1991
- High-affinity binding of water by proteins is similar in air and in organic solventsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
- Subtilisin-catalysed peptide synthesis and transesterification in organic solventsApplied Microbiology and Biotechnology, 1990
- The dependence of the heat of solution of lyophilized solid ribonuclease A on water contentThe Journal of Physical Chemistry, 1978
- Humidity fixed points of binary saturated aqueous solutionsJournal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1977
- Surface Areas of Proteins. III. Adsorption of Water1Journal of the American Chemical Society, 1950
- Propagation of temperature changes through textiles in humid atmospheresTransactions of the Faraday Society, 1940