Enzymatic Detection of Urinary Steroid-17β-Glucuronides after Gel Filtration
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 5 (4) , 743-749
- https://doi.org/10.1080/01483918208060582
Abstract
An enzymatic detection of [human] urinary steroid-17.beta.-glucuronides is described. The principle of the method is as follows; after gel filtration with Sephadex G-25 .beta.-glucuronidase is added to each effluent fraction and incubated for 20 h at 37.degree. C. After hydrolysis, 3.beta., 17.beta.-hydroxysteroid dehydrogenase is added and incubated for 20 min at 37.degree. C. An absorbance at 500 nm is read against sample of 1st fraction effluent.This publication has 5 references indexed in Scilit:
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