Acute regulation of insulin-like growth factor-I gene expression by growth hormone during adipose cell differentiation.

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Abstract
Insulin‐like growth factor I (IGF‐I) is a mitogenic polypeptide that is thought to play, under the control of growth hormone, a role in fetal development as well as post‐natally. The direct effect of growth hormone on the regulation of the expression of IGF‐I gene was examined in adipose Ob1771 cells. Growth hormone regulates the abundance of multiple species of IGF‐I mRNAs of 15, 7.5, 1.5 and 0.8 kb in a differentiation‐dependent manner. The regulation of IGF‐I gene expression is strikingly rapid (less than 2 h), reversible and takes place primarily at transcriptional level. Thus growth hormone can increase the cellular content of IGF‐I mRNA encoding for a protein which could be involved in a paracrine/autocrine action during adipose tissue development.