Ultraviolet inactivation of murine leukemia and sarcoma viruses

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Abstract
The ultraviolet (UV) sensitivity of Friend leukemia virus and a murine sarcoma virus (MSV) was determined. The inactivation dose D10 was 2,000 to 3,000 ergs/mm2 for both. This value is close to the one obtained with Rous sarcoma virus, 3,000 ergs/mm2 (Goldé et al., 1961). MSV produced by the cells incubated in 5‐fluorouracil (5 × 10−3 M) was significantly more UV sensitive than control MSV, suggesting the sensitization of the viral nucleic acid by the base analogue.The virus surviving UV irradiation showed a prolonged latent period, but it was due to the interference of the inactivated virions with the normal growth of surviving virions.When UV‐irradiated MSV was titrated on the cells previously irradiated with UV, the slope of the inactivation curve was steeper than when the virus was titrated on the normal cells. This may suggest the complementation of the UV‐damaged viral genome by the corresponding homologous host genome.