cDNA cloning of a novel cdc2+/CDC28‐related protein kinase from rice
- 11 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (1) , 149-152
- https://doi.org/10.1016/0014-5793(91)80271-4
Abstract
A cDNA clone, named R2, has been isolated by screening a rice cell cDNA library with a redundant oligonucleotide probe derived from the conserved ATP binding site of cdc2+/CDC28 protein kinases. The cDNA contained the entire coding sequence for a 424 amino acid polypeptide with a molecular mass of 47.6 kDa. The R2 mRNA, 2.1 kb in size, was expressed in both cultured rice cells and rice seedings at similar levels. The predicted R2 protein has canonical motifs for ATP binding and catalysis, and is significantly homologous (up to 47%) to members of the cdc2+/ CDC28 subfamily of serine/threonine protein kinase. The R2 protein is a novel member of the subfamily.Keywords
This publication has 20 references indexed in Scilit:
- Putative protein kinase product of the Drosophila segment-polarity gene zeste-white3Nature, 1990
- Driving the cell cycle: M phase kinase, its partners, and substratesCell, 1990
- Universal control mechanism regulating onset of M-phaseNature, 1990
- Salt-induced accumulation of 26 and 27 kD proteins in cultured cells of rice plantSoil Science and Plant Nutrition, 1990
- A homolog of the cell cycle control protein p34cdc2 participates in the division cycle of Chlamydomonas, and a similar protein is detectable in higher plants and remote taxa.Plant Cell, 1989
- A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiaeCell, 1989
- Reversible tyrosine phosphorylation of cdc2: Dephosphorylation accompanies activation during entry into mitosisCell, 1989
- Diversity and organization of human T cell receptor delta variable gene segments.The Journal of Experimental Medicine, 1989
- The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic DomainsScience, 1988
- Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2Nature, 1987